4IS4
The glutamine synthetase from the dicotyledonous plant M. truncatula is a decamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 99.335, 101.672, 188.084 |
Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
Resolution | 96.511 - 2.350 |
R-factor | 0.1767 |
Rwork | 0.175 |
R-free | 0.21660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d3a |
RMSD bond length | 0.008 |
RMSD bond angle | 1.165 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.000 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.077 | 0.338 |
Number of reflections | 149655 | |
<I/σ(I)> | 19.2 | 4 |
Completeness [%] | 99.0 | 96.8 |
Redundancy | 6.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 287 | 0.12 M triammonium citrate, 9.2% PEG3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 287K |