4IS1
Crystal structure of ZNF217 bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-10-07 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.670, 40.980, 52.040 |
| Unit cell angles | 90.00, 97.21, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.23873 |
| Rwork | 0.237 |
| R-free | 0.26214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f2j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.840 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 | 0.898 |
| Number of reflections | 15360 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.01M ZINC CHLORIDE, 0.1M MES, 20% PEG 6000 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
