4IQZ
The crystal structure of a large insert in RNA polymerase (RpoC) subunit from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9802 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.060, 65.630, 81.292 |
| Unit cell angles | 90.00, 108.92, 90.00 |
Refinement procedure
| Resolution | 35.987 - 2.100 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.24050 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.198 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.104 | 0.658 |
| Number of reflections | 58175 | |
| <I/σ(I)> | 13.85 | 2.79 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 277 | 0.2 M KCl, 0.04 M Tris pH 7.9, 0.1 M Phosphate-Citrate, 1.6 M Sodium di-Hydrogen Phosphate, 0.4 M di-Potassium Hydrogen Phosphate, 0.05 M Sodium Iodide , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
