4IQA
Crystal Structure Analysis of the E228L Mutant of Human CLIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.768, 71.938, 83.074 |
| Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
| Resolution | 36.120 - 2.490 |
| R-factor | 0.2485 |
| Rwork | 0.245 |
| R-free | 0.30370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.399 |
| Data reduction software | SAINT (V7.68A) |
| Data scaling software | SAINT (V7.68A) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.120 | 5.355 | 2.575 |
| High resolution limit [Å] | 2.486 | 4.251 | 2.486 |
| Number of reflections | 17255 | ||
| <I/σ(I)> | 19.841 | ||
| Completeness [%] | 99.0 | ||
| Redundancy | 11.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M NaCl, 0.1 M HEPES 25% (w/v) PEG 3350, 0.02% azide, 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
