4IQ8

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase 3 from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-06-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9792
Spacegroup name	I 41 2 2
Unit cell lengths	116.129, 116.129, 119.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.490
R-factor	0.2167
Rwork	0.214
R-free	0.27358
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pym
RMSD bond length	0.010
RMSD bond angle	1.182
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.490	2.490
Rmerge		0.459
Number of reflections	14571
<I/σ(I)>		7.71
Completeness [%]	99.7	100
Redundancy		10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	287	12% PEG 3350, 0.1M sodium malonate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K