4IPY

HIV capsid C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Collection date	2012-09-28
Wavelength(s)	0.9725
Spacegroup name	P 1
Unit cell lengths	40.141, 43.578, 55.189
Unit cell angles	74.02, 74.21, 69.77

Refinement procedure

Resolution	19.700 - 1.640
R-factor	0.1779
Rwork	0.176
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a8o
RMSD bond length	0.006
RMSD bond angle	1.074
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: dev_1261))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.700
High resolution limit [Å]	1.640
Rmerge	0.046
Number of reflections	40722
<I/σ(I)>	22.6
Completeness [%]	95.3
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	reservoir contained 0.2 M magnesium formate dihydrate (pH 7.0) and 20% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K