4IPV

Crystal structure of Pseudomonas aeruginosa (strain: PAO1) type IV minor pilin FimU in space group P65

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 65
Unit cell lengths	40.687, 40.687, 272.922
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.236 - 1.400
R-factor	0.1655
Rwork	0.164
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ipu
RMSD bond length	0.005
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.044	0.354
Number of reflections	49829
<I/σ(I)>	53.8	5.9
Completeness [%]	99.4	93.9
Redundancy	15.4	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.2 M sodium chloride, 0.1 M Tris-HCl, 25% (w/v) PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K