4IPE
Crystal structure of mitochondrial Hsp90 (TRAP1) with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.977, 96.362, 125.153 |
| Unit cell angles | 90.00, 134.34, 90.00 |
Refinement procedure
| Resolution | 26.240 - 2.289 |
| R-factor | 0.1811 |
| Rwork | 0.180 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1214)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.060 |
| Number of reflections | 67287 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 296 | 0.2M Sodium Malonate pH 6.6, 18% (v/v) PEG 3350, 20uM hexamine cobalt, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
