4IPA
Structure of a thermophilic Arx1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2008-07-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 191.998, 193.316, 70.913 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.176 - 2.300 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q8k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.072 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.400 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.127 | 0.530 |
| Number of reflections | 118103 | |
| <I/σ(I)> | 13.7 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Lithiumacetate, 2.2M Amminiumsulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
