4IOP
Crystal structure of NKp65 bound to its ligand KACL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SIEMENS-NICOLET |
| Wavelength(s) | 1.502 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 74.640, 74.640, 271.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.2) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.962 | 3.280 | |
| High resolution limit [Å] | 3.200 | 14.310 | 3.200 |
| Rmerge | 0.091 | 0.022 | 0.479 |
| Number of reflections | 6745 | ||
| <I/σ(I)> | 33.15 | 89.71 | 5.81 |
| Completeness [%] | 99.7 | 91.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | PEG 1000, NaCl, pH 6.0, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
