4IOL

N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with ADP/ZD9 and XPO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Spacegroup name	H 3 2
Unit cell lengths	160.370, 160.370, 258.074
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	122.300 - 2.563
R-factor	0.19442
Rwork	0.190
R-free	0.27916
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3SIN
RMSD bond length	0.010
RMSD bond angle	1.560
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	122.300
High resolution limit [Å]	2.560
Rmerge	0.049
Number of reflections	79627
<I/σ(I)>	9.4
Completeness [%]	91.1
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	18 to 22% (w/v) PEG 6-8K, 0.2M AS, 1 mM DTT, 75 mM KMB pH 7.0-8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K