4IOK

N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with ADP, XPO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	100
Spacegroup name	P 21 21 2
Unit cell lengths	91.170, 212.976, 53.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.490 - 2.497
R-factor	0.17349
Rwork	0.164
R-free	0.25876
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3RBO
RMSD bond length	0.011
RMSD bond angle	1.616
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.490	2.590
High resolution limit [Å]	2.497	2.497
Rmerge	0.085
Number of reflections	37221
Completeness [%]	91.5	64.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	38 to 46% (w/v) saturated ammonium sulfate (AS), 1 mM DTT, and 1 to 3.5% (w/v) PEG 1000 or PEG 1450, in 50 - 75 mM KMB (pH 7.0 - 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K