4IOJ
N10-formyltetrahydrofolate synthetase from Moorella thermoacetica with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 100 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 161.203, 161.203, 256.915 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 122.670 - 2.202 |
| R-factor | 0.18093 |
| Rwork | 0.178 |
| R-free | 0.23302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3PZX |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 122.670 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.076 |
| Number of reflections | 64918 |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 99.9 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 38 to 46% (w/v) saturated ammonium sulfate (AS), 1 mM DTT, and 1 to 3.5% (w/v) PEG 1000 or PEG 1450, in 50-75 mM KMB (pH 7.0 - 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
