4IOC

Crystal structure of compound 4f bound to large ribosomal subunit (50S) from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	169.640, 408.490, 692.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.600
R-factor	0.2003
Rwork	0.198
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	autoBUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	243125
Completeness [%]	89.4	71.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP