4INA
Crystal Structure of the Q7MSS8_WOLSU protein from Wolinella succinogenes. Northeast Structural Genomics Consortium Target WsR35
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.639, 95.490, 104.427 |
| Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
| Resolution | 45.061 - 2.488 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.251 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.087 | 0.474 |
| Number of reflections | 57476 | |
| <I/σ(I)> | 10.6 | 2.6 |
| Completeness [%] | 96.9 | 97.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 6.5 | 277 | 20% PEG MME 5000, 0.1M BIS-TRIS, pH 6.5, Microbatch crystallization under oil, temperature 277K |
