4IN0
Crystal Structure of human splicing factor dim2/TXNL4B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.325, 63.640, 51.148 |
| Unit cell angles | 90.00, 92.29, 90.00 |
Refinement procedure
| Resolution | 30.567 - 1.327 |
| R-factor | 0.1303 |
| Rwork | 0.128 |
| R-free | 0.18490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xbs |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.189 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.567 |
| High resolution limit [Å] | 1.327 |
| Number of reflections | 56401 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% PEG8000, 0.2 M magnesium acetate tetrahydate, 0.1 M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
