4IM6
LRR domain from human NLRP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.690, 84.690, 130.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.800 - 1.650 |
| R-factor | 0.13691 |
| Rwork | 0.134 |
| R-free | 0.18253 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z7x |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.271 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.038 | 0.376 |
| Number of reflections | 33885 | |
| <I/σ(I)> | 51.6 | 9.71 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 19.8 | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris pH 8.0, 0.02 M MgCl2, 25 % (w/v) PAA 5100, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
