4ILH
Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-17 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.890, 76.760, 91.860 |
Unit cell angles | 90.00, 105.88, 90.00 |
Refinement procedure
Resolution | 33.953 - 1.850 |
R-factor | 0.1703 |
Rwork | 0.168 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sbt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.157 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.080 | 0.880 |
Number of reflections | 45500 | |
<I/σ(I)> | 16.1 | 2 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1 M Tris-HCl, pH 8.0, 19 % (w/v) PEG 4000 and 100 mM KCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K |