4ILE

Structure of human ADP-ribosylation factor-like 8A binding to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9724
Spacegroup name	P 21 21 2
Unit cell lengths	118.032, 37.374, 39.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.300 - 2.676
R-factor	0.1854
Rwork	0.181
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h18
RMSD bond length	0.009
RMSD bond angle	1.285
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.315	2.740
High resolution limit [Å]	2.676	2.680
Rmerge	0.049	0.303
Number of reflections	5242
<I/σ(I)>		1.98
Completeness [%]	98.1	87.7
Redundancy	6.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	16% PEG 4000, 200mM NaCl, 100mM Tris-HCL (pH7.4), VAPOR DIFFUSION, SITTING DROP, temperature 293K