4IL8
Crystal structure of an H329A mutant of p. aeruginosa PMM/PGM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-10-22 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.962, 72.906, 93.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.380 - 1.800 |
| R-factor | 0.18753 |
| Rwork | 0.186 |
| R-free | 0.21932 |
| Structure solution method | refinement |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.503 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.380 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.491 |
| Number of reflections | 45415 | |
| <I/σ(I)> | 15.7 | 3.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.11 | 6.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Na,K tartrate 1.2 M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
