4IL3

Crystal Structure of S. mikatae Ste24p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	90
Detector technology	CCD
Collection date	2012-08-19
Detector	MARMOSAIC 300
Wavelength(s)	1.27
Spacegroup name	P 65
Unit cell lengths	143.530, 143.530, 197.024
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.449 - 3.102
R-factor	0.2706
Rwork	0.270
R-free	0.29260
Structure solution method	SAD, MOLECULAR REPLACEMENT
Starting model (for MR)	4aw6
RMSD bond length	0.004
RMSD bond angle	0.778
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.131	0.052	0.435
Number of reflections	26917
<I/σ(I)>	5	43.3	2.29
Completeness [%]	64.8	99.9	17.5
Redundancy	5.5	6.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	290	10% PEG 20,000, 20% PEG 550MME, 5 mM 1,6-hexanediol, 5 mM 1-butanol, 5 mM (RS)-1,2-propanediol, 5 mM 2-propanol, 5 mM 1,4-butanediol, 5 mM 1,3-propanediol, 100 mM MOPS / Na-HEPES pH 7.5, vapor diffusion, temperature 290K