4IKF
PFV intasome with inhibitor MB-76
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 159.070, 159.070, 123.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.854 - 3.400 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.123 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC ((CCP4)) |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 3.555 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 28606 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 1.2 M (NH4)2SO4, 25% (v/v) glycerol, 4.6% (w/v) 1,6-hexanediol, 50 mM MES-NaOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
