4IKE

Crystal Structure of a partly open ATP-lid of liganded Adenylate kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999800
Spacegroup name	P 1 21 1
Unit cell lengths	56.370, 61.570, 58.890
Unit cell angles	90.00, 94.33, 90.00

Refinement procedure

Resolution	33.040 - 1.480
R-factor	0.1629
Rwork	0.161
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.141
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	AutoBuild
Refinement software	PHENIX ((phenix.refine: dev_1039))

Data quality characteristics

	Overall
Low resolution limit [Å]	34.820
High resolution limit [Å]	1.480
Rmerge	0.076
Number of reflections	66831
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	295	pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K