4IJS

Crystal structure of nucleocapsid protein encoded by the prototypic member of orthobunyavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-01
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	I 4 2 2
Unit cell lengths	106.140, 106.140, 485.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.746 - 3.200
R-factor	0.2229
Rwork	0.220
R-free	0.27620
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.548
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	23406
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1