4IJD
Crystal structure of methyltransferase domain of human PR domain-containing protein 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.734, 48.782, 78.713 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 36.190 - 2.150 |
| R-factor | 0.1991 |
| Rwork | 0.196 |
| R-free | 0.26400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.195 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 5.860 | 2.150 |
| Rmerge | 0.093 | 0.066 | 0.804 |
| Number of reflections | 21804 | ||
| <I/σ(I)> | 31.1 | ||
| Completeness [%] | 99.9 | 99 | 99.7 |
| Redundancy | 6.3 | 6.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 23% PEG 3350, 0.2 M AMMONIUM ACETATE, 0.1 M BisTris pH5.5, vapor diffusion hanging drop, temperature 291K |
