4IHN

High Resolution Insulin by Langmuir-Blodgett Modified Hanging Drop Vapour Diffusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9395
Spacegroup name	I 21 3
Unit cell lengths	78.340, 78.340, 78.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.390 - 1.160
R-factor	0.2343
Rwork	0.233
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.035
RMSD bond angle	2.329
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.395	19.585	1.220
High resolution limit [Å]	1.158	3.660	1.160
Rmerge		0.100	0.138
Rmeas		0.125	0.169
Rpim		0.074	0.094
Total number of observations		407	11155
Number of reflections	26153
<I/σ(I)>	8.9	9.4	6.4
Completeness [%]	93.9	18.3	99.7
Redundancy	2.8	2.2	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1