4IHJ

Crystal structure of tubulin-stathmin-TTL-ADP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.999950
Spacegroup name	P 21 21 21
Unit cell lengths	104.520, 157.310, 180.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.100 - 2.000
R-factor	0.1702
Rwork	0.168
R-free	0.21740
Structure solution method	Difference Fourier
Starting model (for MR)	4i4t
RMSD bond length	0.008
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.137	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	198918
<I/σ(I)>	13.3	1.17
Completeness [%]	99.1	92.5
Redundancy	26.8	25.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	4-10% PEG 4000, 4-6% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 100 mM MES/Imidazole, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K