4IHB

X-RAY STRUCTURE OF THE canonical C2A DOMAIN FROM HUMAN DYSFERLIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	90
Detector technology	CCD
Detector	Mercury 3
Wavelength(s)	1.5418
Spacegroup name	I 1 2 1
Unit cell lengths	102.413, 70.670, 118.310
Unit cell angles	90.00, 113.39, 90.00

Refinement procedure

Resolution	33.902 - 2.044
R-factor	0.2041
Rwork	0.200
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dmh
RMSD bond length	0.008
RMSD bond angle	1.347
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.000	2.150
High resolution limit [Å]	2.040	2.040
Rmerge	0.073	0.415
Number of reflections	49251
<I/σ(I)>		0.415
Completeness [%]	99.3	99.5
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	284	2M Sodium Formate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 284K
1	VAPOR DIFFUSION, HANGING DROP	5.4	284	2M Sodium Formate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 284K