4IH1
Crystal structure of Karrikin Insensitive 2 (KAI2) from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-21 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.335, 56.013, 53.525 |
| Unit cell angles | 90.00, 116.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.1797 |
| Rwork | 0.179 |
| R-free | 0.19270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wom |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.099 | 0.078 | 0.626 |
| Number of reflections | 39492 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 99.9 | 99.9 | 99.6 |
| Redundancy | 7.1 | 6.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG400, ammonium sulfate, pH 7.5, vapor diffusion, temperature 298K |
