4IGQ

Histone H3 Lysine 4 Demethylating Rice JMJ703 in complex with methylated H3K4 substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 1W2B
Synchrotron site	BSRF
Beamline	1W2B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-03
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 63
Unit cell lengths	91.425, 91.425, 75.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.588 - 2.350
R-factor	0.1837
Rwork	0.181
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oq6
RMSD bond length	0.008
RMSD bond angle	1.225
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	15108
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	potassium phosphate monobasic, 25% (w/v) polyethylene glycol 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K