4IGN
2.32 Angstrom X-ray Crystal structure of R47A mutant of human ACMSD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2012-12-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.142, 101.686, 232.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.329 |
| R-factor | 0.2114 |
| Rwork | 0.208 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.785 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.329 | 2.329 |
| Number of reflections | 89705 | |
| Completeness [%] | 97.2 | 73.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
