4IEI
Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6 complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-11-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00318 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.468, 63.213, 149.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.783 - 2.090 |
| R-factor | 0.1769 |
| Rwork | 0.175 |
| R-free | 0.21090 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4idh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.162 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.120 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.064 | 0.740 |
| Number of reflections | 32795 | |
| <I/σ(I)> | 13.7 | 1.6 |
| Completeness [%] | 98.8 | 91.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM Tris, 15% ethanol , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
