4IEG
Structure and interactions of the RNA-dependent RNA polymerase from bacteriophage phi12 (P1 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.874, 94.470, 96.481 |
| Unit cell angles | 75.16, 63.11, 83.79 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.22025 |
| Rwork | 0.217 |
| R-free | 0.27836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gzk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.202 |
| Number of reflections | 144893 | |
| <I/σ(I)> | 17.8 | 2.9 |
| Completeness [%] | 92.6 | 87.1 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Mg formate 20% PEG 3350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
