4IEE
Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6 complexed with ATP-r-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-04-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.101, 63.142, 149.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.748 - 1.890 |
| R-factor | 0.1676 |
| Rwork | 0.166 |
| R-free | 0.20760 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4idh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.045 | 0.363 |
| Number of reflections | 43101 | |
| <I/σ(I)> | 29.92 | 3.29 |
| Completeness [%] | 97.5 | 91.7 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM Tris, 15% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
