4IDQ
human atlastin-1 1-446, N440T, GDPAlF4-
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 128.993, 267.084, 62.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.139 - 2.295 |
| R-factor | 0.2071 |
| Rwork | 0.206 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q5d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.295 | 2.295 |
| Number of reflections | 92313 | |
| <I/σ(I)> | 35.6 | 10.5 |
| Completeness [%] | 95.3 | 81.1 |
| Redundancy | 14.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.2 M lithium citrate tribasic tetrahydrate, 20% PEG3350, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
