4IDH
Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2011-10-21 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.03304 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.790, 63.440, 150.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.859 - 1.690 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | nuclease domain of gp2 of bacterial virus sf6 and ATPase domain of gp17 of T4 bacteriophage |
RMSD bond length | 0.007 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.720 | 1.780 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.115 | 0.549 |
Number of reflections | 62415 | |
<I/σ(I)> | 7.1 | 2.3 |
Completeness [%] | 99.3 | 100 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM Tris, 15% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |