4ICB

PROLINE CIS-TRANS ISOMERS IN CALBINDIN D9K OBSERVED BY X-RAY CRYSTALLOGRAPHY

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	56.130, 43.000, 29.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 1.600
R-factor	0.188
Rwork	0.188
RMSD bond length	0.024
RMSD bond angle	0.043 *
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.067 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop		15 (mM)
4	1	drop	sodium sulphate	80 (%sat)