4IBX
Crystal structure of stabilized TEM-1 beta-lactamase variant v.13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97300 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.900, 239.000, 159.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.680 - 2.680 |
| R-factor | 0.23394 |
| Rwork | 0.232 |
| R-free | 0.26529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zg4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.699 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.000 |
| High resolution limit [Å] | 2.680 |
| Number of reflections | 43737 |
| Completeness [%] | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 9%(wt/vol) polyethylene glycol (PEG) 8000, 100 mM MES pH 6.2, and 200mM Ca(OAc)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
