4IBG
Ebola virus VP35 bound to small molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.474, 65.937, 72.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.759 - 1.413 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.162 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.430 |
| High resolution limit [Å] | 1.410 | 3.830 | 1.410 |
| Rmerge | 0.100 | 0.051 | 0.740 |
| Number of reflections | 47377 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 98.8 | 97.4 | 87.3 |
| Redundancy | 7.6 | 7.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM sodium citrate, pH5.5, 15% PEG3350, 10%DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
