4IB3

Structure of cAMP dependent protein kinase A in complex with ADP, phosphorylated peptide pSP20, and no metal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-04-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	57.541, 79.309, 98.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.182
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.191
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.200
Number of reflections	20083
Completeness [%]	86.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	100 mM MES pH 6.5, 5 mM DTT, 0.3M EDTA, 15-20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K