4IAG

Crystal structure of ZbmA, the zorbamycin binding protein from Streptomyces flavoviridis

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-03
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97929
Spacegroup name	P 65 2 2
Unit cell lengths	38.787, 38.787, 268.524
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.590 - 1.900
R-factor	0.1913
Rwork	0.189
R-free	0.23140
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.836
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.068	0.057	0.434
Number of reflections	10484
<I/σ(I)>	11.2
Completeness [%]	98.5	97.1	87.5
Redundancy	9.7	8.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	289	protein subject to reductive isopropylation, 0.8M Ammonium Sulfate, 0.1M Sodium Sitrate: HCl pH 4, VAPOR DIFFUSION, SITTING DROP, temperature 289K