4I9Q
Crystal structure of the ternary complex of the D714A mutant of RB69 DNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.781, 119.412, 146.016 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 38.403 - 2.300 |
| R-factor | 0.2537 |
| Rwork | 0.252 |
| R-free | 0.28710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1227)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.117 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 110655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 10% PEG 8000, 0.2 M (NH4)2SO4 and 0.1 M sodium citrate (pH 5.6), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
