4I96

Crystal structure of the N-terminal two domains of the skeletal muscle ryanodine receptor (rabbit RyR1) residues 217-536

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Detector technology	CCD
Collection date	2010-05-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 31 2 1
Unit cell lengths	68.449, 68.449, 131.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.090 - 2.730
R-factor	0.22462
Rwork	0.224
R-free	0.24132
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xoa
RMSD bond length	0.011
RMSD bond angle	1.314
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.950	3.260	2.800
High resolution limit [Å]	2.730	3.150	2.730
Number of reflections	10067
Completeness [%]	100.0	100	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8		pH 8.0, EVAPORATION