4I8A
Alanine-glyoxylate aminotransferase variant S187F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.892, 101.564, 116.749 |
| Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
| Resolution | 18.070 - 2.900 |
| R-factor | 0.25273 |
| Rwork | 0.251 |
| R-free | 0.27813 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0c |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.466 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.090 |
| High resolution limit [Å] | 2.620 |
| Rmerge | 0.195 |
| Number of reflections | 53733 |
| <I/σ(I)> | 4.5 |
| Completeness [%] | 96.7 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES (pH 7.5), 0.1 M LiSO4, 23 % [w/w] PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
