4I7W

Agrobacterium tumefaciens DHDPS with lysine and pyruvate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Wavelength(s)	0.9536
Spacegroup name	C 2 2 21
Unit cell lengths	89.090, 122.780, 129.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.300
R-factor	0.17192
Rwork	0.172
R-free	0.18647
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.058
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.300
Number of reflections	39292
Completeness [%]	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.17 M lithium sulfate monohydrate, 0.085 M Tris pH 8.5, 25.5 % (w/v) PEG 4000, 15% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K