4I70
Crystal structure of the Trypanosoma brucei Inosine-Adenosine-Guanosine nucleoside hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 48.040, 48.040, 216.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.000 - 1.600 |
| R-factor | 0.1438 |
| Rwork | 0.142 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IAGNH-363 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.541 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.037 | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 10.000 | 1.600 |
| Rmerge | 0.099 | 0.036 | 0.389 |
| Rmeas | 0.038 | 0.418 | |
| Number of reflections | 38235 | 181 | 6215 |
| <I/σ(I)> | 22.52 | 58.2 | 5.94 |
| Completeness [%] | 99.7 | 98.4 | 99.3 |
| Redundancy | 9.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.8 | 292 | 0.2M ammonium acetate, 18% PEG3350, pH 4.8, VAPOR DIFFUSION, temperature 292K |
