4I6Z
Crystal structure of the transcriptional regulator TM1030 with 24bp DNA oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.008, 45.954, 82.510 |
| Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
| Resolution | 33.230 - 3.200 |
| R-factor | 0.2033 |
| Rwork | 0.200 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2id6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.651 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
| Rmerge | 0.095 | 0.053 | 0.519 |
| Number of reflections | 11502 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 4.1 | 3.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 310 | 0.1M Tris, 20% PEG 4000, pH 8.0, VAPOR DIFFUSION, temperature 310K |
