4I69
Crystal structure of the K463A mutant of the RRM domain of RNA helicase HERA from T. thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0097 |
| Spacegroup name | P 31 |
| Unit cell lengths | 49.221, 49.221, 78.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.627 - 1.790 |
| R-factor | 0.2004 |
| Rwork | 0.195 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i31 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1223)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.800 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.092 | 0.587 |
| Number of reflections | 19275 | |
| <I/σ(I)> | 8.9 | 1.1 |
| Completeness [%] | 95.6 | |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
