4I68

Crystal structure of the R444A / R449A double mutant of the HERA RNA helicase RRM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	25.160, 46.030, 59.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.432 - 1.630
R-factor	0.1818
Rwork	0.179
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i31
RMSD bond length	0.006
RMSD bond angle	1.149
Data reduction software	XDS ((VERSION September 26)
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1223))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	1.720
High resolution limit [Å]	1.630	1.630
Rmerge	0.153	0.635
Number of reflections	9118
<I/σ(I)>	8.49	1.37
Completeness [%]	99.4	97.5
Redundancy	6.38	3.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	26% PEG8000, 0.1 M zinc acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K