4I64

3-hydroxy-3-methylglutaryl Coenzyme A reductase from Pseudomonas mevalonii, a high resolution native structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	I 41 3 2
Unit cell lengths	225.613, 225.613, 225.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.620 - 1.750
R-factor	0.19914
Rwork	0.198
R-free	0.24630
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	0.928
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.720	1.860
High resolution limit [Å]	1.750	1.750
Number of reflections	94406
Completeness [%]	98.2	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.2M ammonium sulfate, 75mM sodium citrate, 100 mM sodium ADA microseeding, pH 293, VAPOR DIFFUSION, SITTING DROP, temperature 6.5K